NMR metabolic profiling of Aspergillus nidulans to monitor drug and protein activity.
نویسندگان
چکیده
We describe a general protocol for using comparative NMR metabolomics data to infer in vivo efficacy, specificity and toxicity of chemical leads within a drug discovery program. The methodology is demonstrated using Aspergillus nidulans to monitor the activity of urate oxidase and orotidine-5'-phosphate decarboxylase and the impact of 8-azaxanthine, an inhibitor of urate oxidase. 8-azaxanthine is shown to inhibit A. nidulans hyphal growth by in vivo inactivation of urate oxidase.
منابع مشابه
Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
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عنوان ژورنال:
- Journal of proteome research
دوره 5 8 شماره
صفحات -
تاریخ انتشار 2006